Premium
Synthesis, quality control, and bio‐evaluation of 99m Tc‐cyclophosphamide
Author(s) -
Bokhari Tanveer Hussain,
Roohi Samina,
Hina Saira,
Akbar Muhammad Usman,
Sohaib Muhammad,
Iqbal Munawar
Publication year - 2018
Publication title -
chemical biology and drug design
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.59
H-Index - 77
eISSN - 1747-0285
pISSN - 1747-0277
DOI - 10.1111/cbdd.13107
Subject(s) - quality (philosophy) , cyclophosphamide , chemistry , radiochemistry , medicine , physics , quantum mechanics , chemotherapy
Cancer is found to be the leading cause of death worldwide characterized by uncontrolled cell division. Nuclear medicines imaging using radiopharmaceuticals have pronounced potential for the diagnosis and treatment of cancers. Cyclophosphamide ( CPH ) is an antineoplastic drug which targets selectively cancer cells. In the present work, labeling of CPH with Tc‐99m is performed for diagnostic purpose, which gave labeling yield as high as 99% using 20 μg SnCl 2 ·2H 2 O, 200 μg of ligand at pH 7 for 10 min reaction time at room temperature. The characterization of the prepared complex was performed using ITLC , electrophoresis, and HPLC . In vitro stability was analyzed in the presence of human serum at 37°C which has maximum value of 94 ± 0.5. The biodistribution studies of 99m Tc‐ CPH were performed in normal and tumor bearing Swiss Webster mice. The high accumulation of 99m Tc‐ CPH was observed in liver and tumours respectively at 4 hr after injection. Biodistribution results revealed that 99m Tc‐ CPH may be a potential tumour diagnostic agent simultaneously with chemotherapy.