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Live cell imaging of bacterial cells: Pyrenoylpyrrole‐based fluorescence labeling
Author(s) -
Arun Divakar Mathiyazhagan,
Shanmugam Sivakumar
Publication year - 2017
Publication title -
chemical biology and drug design
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.59
H-Index - 77
eISSN - 1747-0285
pISSN - 1747-0277
DOI - 10.1111/cbdd.12978
Subject(s) - fluorescence , chalcone , cytotoxicity , absorbance , chemistry , bacteria , chromophore , pyrene , bacterial cell structure , fluorescence lifetime imaging microscopy , combinatorial chemistry , biochemistry , photochemistry , stereochemistry , organic chemistry , chromatography , biology , in vitro , physics , quantum mechanics , genetics
A novel substituted pyrenoylpyrroles was synthesized by the reaction of pyrenoyl chalcone, Tos MIC and methyl iodide under mild condition. All the synthesized compounds were screened for their bioactivity, and the MIC was determined, among which few compounds showed moderate antibacterial activity toward Gram‐positive as well as Gram‐negative bacteria. Further, cytotoxicity assay ascertained that these compounds were non‐toxic to mammalian cells as well. The pyrene chromophore in the synthesized compounds ( 3a–e ) and ( 5a–e ) is responsible for the good photophysical properties which have an absorbance at λ 340 nm and emission at λ 410 nm. Hence, two of the selected novel synthesized compounds with non‐cytotoxic nature prospected for bio‐imaging of bacterial cells using high‐content screening analysis show that the molecule is suitable for microbial imaging in pathological diagnostic studies.