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Mannich bases of 1,2,4‐triazole‐3‐thione containing adamantane moiety: Synthesis, preliminary anticancer evaluation, and molecular modeling studies
Author(s) -
Milošev Milorad Z.,
Jakovljević Katarina,
Joksović Milan D.,
Stanojković Tatjana,
Matić Ivana Z.,
Perović Milka,
Tešić Vesna,
Kanazir Selma,
Mladenović Milan,
Rodić Marko V.,
Leovac Vukadin M.,
Trifunović Snežana,
Marković Violeta
Publication year - 2017
Publication title -
chemical biology and drug design
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.59
H-Index - 77
eISSN - 1747-0285
pISSN - 1747-0277
DOI - 10.1111/cbdd.12920
Subject(s) - adamantane , cytotoxicity , chemistry , docking (animal) , moiety , stereochemistry , molecular model , apoptosis , cell cycle , cell culture , k562 cells , cell cycle checkpoint , triazole , combinatorial chemistry , biochemistry , in vitro , biology , organic chemistry , medicine , nursing , genetics
A series of 18 novel N ‐Mannich bases derived from 5‐adamantyl‐1,2,4‐triazole‐3‐thione was synthesized and characterized using NMR spectroscopy and X‐ray diffraction technique. All derivatives were evaluated for their anticancer potential against four human cancer cell lines. Several tested compounds exerted good cytotoxic activities on K562 and HL ‐60 cell lines, along with pronounced selectivity, showing lower cytotoxicity against normal fibroblasts MRC ‐5 compared to cancer cells. The effects of compounds 5b , 5e, and 5j on the cell cycle were investigated by flow cytometric analysis. It was found that these compounds cause the accumulation of cells in the subG1 and G1 phases of the cell cycle and induce caspase‐dependent apoptosis, while the anti‐angiogenic effects of 5b , 5e, and 5j have been confirmed in EA .hy926 cells using a tube formation assay. Further, the interaction of Bax protein with compound 5b was investigated by means of molecular modeling, applying the combined molecular docking/molecular dynamics approach.