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Synthesis, in vitro evaluation and molecular docking studies of novel coumarin‐isatin derivatives as α‐glucosidase inhibitors
Author(s) -
Wang Guangcheng,
Wang Jing,
He Dianxiong,
Li Xin,
Li Juan,
Peng Zhiyun
Publication year - 2017
Publication title -
chemical biology and drug design
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.59
H-Index - 77
eISSN - 1747-0285
pISSN - 1747-0277
DOI - 10.1111/cbdd.12867
Subject(s) - isatin , coumarin , acarbose , chemistry , stereochemistry , docking (animal) , enzyme , ic50 , active site , in vitro , combinatorial chemistry , biochemistry , organic chemistry , medicine , nursing
This study synthesized a series of novel coumarin‐isatin derivatives and evaluated them for α‐glucosidase inhibitory activity. The majority of the screened compounds exhibited excellent inhibition activities with IC 50 values of 2.56 ± 0.08–268.79 ± 3.04 μ m , when compared to acarbose. Among the newly derivatives, compound 5p was found to be the most active compound in the library of coumarin‐isatin derivatives. Furthermore, enzyme kinetic studies showed that compound 5p is a non‐competitive inhibitor with a K i of 2.14 μ m . Molecular docking analysis revealed the existence of hydrophobic and hydrogen interactions between compound 5p and the active site of α‐glucosidase. Our results indicate that coumarin‐isatin derivatives as a new class of α‐glucosidase inhibitors.