Premium
Docetaxel –Chitosan nanoparticles for breast cancer treatment: cell viability and gene expression study
Author(s) -
Mirzaie Zahra H.,
Irani Shiva,
Mirfakhraie Reza,
Atyabi Seyed Mohammad,
Dinarvand Meshkat,
Dinarvand Rassoul,
Varshochian Reyhaneh,
Atyabi Fatemeh
Publication year - 2016
Publication title -
chemical biology and drug design
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.59
H-Index - 77
eISSN - 1747-0285
pISSN - 1747-0277
DOI - 10.1111/cbdd.12814
Subject(s) - docetaxel , cytotoxicity , chemistry , viability assay , chitosan , nanoparticle , zeta potential , cancer cell , in vitro , gene expression , cancer research , microbiology and biotechnology , materials science , cancer , biochemistry , gene , biology , nanotechnology , medicine
Docetaxel acts through the inhibition of tubulin polymerization and reduction in the expression of BCL ‐2 gene. In this study, nanoparticles containing Docetaxel were prepared and their effects on the gene expression levels of BCL ‐2 and BAX genes were investigated. The drug was first conjugated to chitosan, and the nanoparticles were assembled in the presence of hyaluronic acid. Conjugations were confirmed by 1 H‐ NMR , and the obtained nanoparticles were characterized by dynamic light scattering and SEM . Cytotoxicity of the nanoparticles, cellular uptake, and cell death were evaluated. Finally, the effect of nanoparticles on the expression of BAX and BCL ‐2 genes in MCF ‐7 cells were investigated through real‐time PCR . The results revealed that the prepared NP s had spherical shape with narrow size distribution of <200 nm with positive zeta potentials. In vitro cytotoxicity of Cs nanoparticles and free Docetaxel investigations revealed that increasing the treatment time with nanoparticles led to decrease in the rate of cell viability. BAX and BCL ‐ 2 gene expressions were decreased in nanoparticle‐treated cells in comparison with intact cells, while the BAX / BCL ‐2 ratio was significantly elevated compared with free drug‐treated cells after 72 h. Docetaxel ‐conjugated NP s may offer a promising treatment with low off‐target toxicity for breast cancer.