Premium
Novel Alginate–Chitosan Composite Microspheres for Implant Delivery of Vancomycin and In Vivo Evaluation
Author(s) -
Mao Yimin,
Zhao Ming,
Ge Yongbiao,
Fan Jiang
Publication year - 2016
Publication title -
chemical biology and drug design
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.59
H-Index - 77
eISSN - 1747-0285
pISSN - 1747-0277
DOI - 10.1111/cbdd.12771
Subject(s) - chitosan , in vivo , vancomycin , microsphere , particle size , composite number , drug delivery , emulsion , chemistry , biomedical engineering , chromatography , materials science , chemical engineering , staphylococcus aureus , composite material , biochemistry , medicine , organic chemistry , genetics , microbiology and biotechnology , bacteria , engineering , biology
In this study, vancomycin loaded alginate–chitosan composite microspheres were developed by emulsion cross‐linking method. The in vitro and vivo characterizations were done to evaluate the feasibility of application. Our experimental results showed that the emulsification cross‐linking technique appeared to be a feasible method for the preparation of alginate–chitosan composite microspheres. The microspheres were spherical in shape and the mean particle size and drug loading were 25.3 ± 5.4 μ m and 18.5 ± 2.3% respectively. A sustained vancomycin release was realized i.e. the amount of cumulative release increased in a time frame of 24 h to reach an amount i.e. ~68%. The model that fit best for vancomycin released from the microspheres was the Higuchi kinetic model with a correlation coefficient r = 0.9996. In vivo results showed that the application of microspheres not only reduced the toxicity, but also maintained effective drug concentration. In addition, no severe signs of epithelial necrosis and sloughing of epithelial cells were detected in histological studies.