Premium
The Truncated Human Telomeric Sequence forms a Hybrid‐Type Intramolecular Mixed Parallel/antiparallel G‐quadruplex Structure in K + Solution
Author(s) -
Liu Yuxia,
Cheng Dengfeng,
Ge Min,
Lin Weizhen
Publication year - 2016
Publication title -
chemical biology and drug design
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.59
H-Index - 77
eISSN - 1747-0285
pISSN - 1747-0277
DOI - 10.1111/cbdd.12740
Subject(s) - antiparallel (mathematics) , g quadruplex , telomere , circular dichroism , telomerase , chemistry , intramolecular force , drug design , protein secondary structure , stereochemistry , crystallography , dna , biochemistry , gene , physics , quantum mechanics , magnetic field
In 80–90% tumor cells, telomerase becomes active and stabilizes the length of telomeres. The formation and stabilization of G‐quadruplexes formed from human telomeric sequences have been proved able to inhibit the activity of telomerase, thus human telomeric G‐quadruplex structure has become a potential target for the development of cancer therapy. Hence, structure of G‐quadruplex formed in K + solution has been an attractive hotspot for further studies. However, the exact structure of human telomeric G‐quadruplex in K + is extremely controversial, this study provides information for the understanding of different G‐quadruplexes. Here, we report that 22nt and 24nt human telomeric sequences form unimolecular hybrid‐type mixed parallel/antiparallel G‐quadruplex in K + solution elucidated utilizing Circular Dichroism, Differential Scanning Calorimetry, and gel electrophoresis. Moreover, individual configuration of these two sequences was speculated in this study. The detailed structure information of the G‐quadruplex formed under physiologically relevant condition is necessary for structure‐based rational drug design.