Premium
Systematic Comparisons of Formulations of Linear Oligolysine Peptides with si RNA and Plasmid DNA
Author(s) -
Kwok Albert,
McCarthy David,
Hart Stephen L.,
Tagalakis Aristides D.
Publication year - 2016
Publication title -
chemical biology and drug design
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.59
H-Index - 77
eISSN - 1747-0285
pISSN - 1747-0277
DOI - 10.1111/cbdd.12709
Subject(s) - transfection , rna , dna , chemistry , plasmid , peptide , cationic polymerization , biophysics , biochemistry , microbiology and biotechnology , biology , gene , polymer chemistry
The effects of lysine peptide lengths on DNA and si RNA packaging and delivery were studied using four linear oligolysine peptides with 8 (K8), 16 (K16), 24 (K24) and 32 (K32) lysines. Oligolysine peptides with 16 lysines or longer were effective for stable monodisperse particle formation and optimal transfection efficiency with plasmid DNA ( pDNA ), but K8 formulations were less stable under anionic heparin challenge and consequently displayed poor transfection efficiency. However, here we show that the oligolysines were not able to package si RNA to form stable complexes, and consequently, si RNA transfection was unsuccessful. These results indicate that the physical structure and length of cationic peptides and their charge ratios are critical parameters for stable particle formation with pDNA and si RNA and that without packaging, delivery and transfection cannot be achieved.