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Knockdown of GPR137 ,G Protein‐coupled receptor 137, Inhibits the Proliferation and Migration of Human Prostate Cancer Cells
Author(s) -
Ren Jizhong,
Pan Xiuwu,
Li Lin,
Huang Yi,
Huang Hai,
Gao Yi,
Xu Hong,
Qu Fajun,
Chen Lu,
Wang Linhui,
Hong Yi,
Cui Xingang,
Xu Danfeng
Publication year - 2016
Publication title -
chemical biology and drug design
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.59
H-Index - 77
eISSN - 1747-0285
pISSN - 1747-0277
DOI - 10.1111/cbdd.12704
Subject(s) - gene knockdown , du145 , gene silencing , small hairpin rna , rna interference , cancer research , cell growth , prostate cancer , cell cycle , biology , signal transduction , microbiology and biotechnology , cell , cancer , chemistry , cell culture , gene , rna , lncap , genetics
GPR137 belongs to the G protein‐coupled receptor family involving the regulation of transmembrane signal transduction that launches pivotal cellular functions. However, its function in prostate cancer (PCa) has been rarely reported. It was found in this study that GPR137 was upregulated in PCa tissues as compared with that in paracancerous tissues. To see whether GPR137 could serve as a potential therapeutic target for PCa, GPR137 was knocked down to verify its biological function in PCa cells. Lentivirus‐introduced short hairpin RNA (shRNA) was designed to silence GPR137 gene. It was found that silencing of GPR137 gene suppressed the proliferation and colony formation of PCa cell lines PC‐3 and DU145. Further study indicated that growth inhibition by GPR137 knockdown was associated with cell cycle arrest at G0/G1 phase. Furthermore, silencing of GPR137 repressed the invasion and migration abilities of PC‐3 cells via downregulating slug and snail and upregulating E‐cadherin. Collectively, these findings imply that GPR137 plays an important role in the occurrence and progression of PCa and may prove to be a potential therapeutic target for the treatment of advanced PCa.