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A Splicing Reporter Tuned to Non‐AG Acceptor Sites Reveals that Luteolin Enhances the Recognition of Non‐canonical Acceptor Sites
Author(s) -
Chiba Masanori,
Ariga Hiroyoshi,
Maita Hiroshi
Publication year - 2016
Publication title -
chemical biology and drug design
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.59
H-Index - 77
eISSN - 1747-0285
pISSN - 1747-0277
DOI - 10.1111/cbdd.12656
Subject(s) - rna splicing , polypyrimidine tract , intron , splice , splice site mutation , genetics , exonic splicing enhancer , gene , protein splicing , biology , chemistry , computational biology , rna
Removal of an intron requires precise recognition of the splice donor and acceptor sites located at the 5′ and 3′ termini of introns. Although the roles of these sequences differ, mutations in both sites easily block normal splicing and produce an aberrant mRNA . For example, many splice‐site mutations occur in patients with inherited diseases. Several approaches have been evaluated to restore expression of a functional protein; however, because of the strict requirement for an AG dinucleotide at the 3′ terminus of a U2‐type intron, no method is available to correct splicing at a mutated sequence. To identify compounds that allow splicing at the non‐ AG acceptor site, in the present study we constructed a reporter gene with a modified polypyrimidine tract. However, the modified polypyrimidine tract mediated splicing at adjacent non‐canonical acceptor sites, including the original mutated site. Further, we show that certain flavones such as luteolin and apigenin enhanced aberrant splicing at the non‐canonical acceptor site of the reporter gene. These results suggest that the reporter gene and luteolin may be useful for further screening to identify molecules that correct aberrant splicing caused by a disease‐associated splice acceptor site mutation.