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Retracted: Long Non‐coding RNA Linc‐ ITGB 1 Knockdown Inhibits Cell Migration and Invasion in GBC ‐ SD /M and GBC ‐ SD Gallbladder Cancer Cell Lines
Author(s) -
Wang Lei,
Zhang Yunjiao,
Lv Wenjie,
Lu Jianhua,
Mu Jiasheng,
Liu Yingbin,
Dong Ping
Publication year - 2015
Publication title -
chemical biology and drug design
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.59
H-Index - 77
eISSN - 1747-0285
pISSN - 1747-0277
DOI - 10.1111/cbdd.12573
Subject(s) - gene knockdown , cancer research , gallbladder cancer , downregulation and upregulation , metastasis , vimentin , epithelial–mesenchymal transition , cell growth , malignancy , long non coding rna , cancer , cell migration , cell , biology , medicine , pathology , cell culture , immunology , immunohistochemistry , genetics , gene , biochemistry
Gallbladder cancer is a highly aggressive malignancy with a low 5‐year survival rate. Despite advances in the molecular understanding of the initiation and progression in gallbladder cancer, treatment modalities such as surgery, radiotherapy, or chemotherapy in advanced cases did not yield promising outcomes. Therefore, it is of great importance to uncover new mechanism underlying gallbladder cancer growth and metastasis. In this study, we identified a differentially expressed long intergenic non‐coding RNA , linc‐ ITGB 1, in a pair of higher and lower metastatic gallbladder cancer cell sublines. Then, the potential role of linc‐ ITGB 1 in gallbladder cancer cell proliferation, migration, and invasion was explored using a lentivirus‐mediated RNA interference system. Functional analysis showed that knockdown of linc‐ ITGB 1 significantly inhibited gallbladder cancer cell proliferation. Moreover, cell migration and invasion were reduced by over twofold in linc‐ ITGB 1 knockdown cells probably due to upregulation of β ‐catenin and downregulation of vimentin, slug, and TCF 8. In conclusion, linc‐ ITGB 1 potentially promoted gallbladder cancer invasion and metastasis by accelerating the process of epithelial‐to‐mesenchymal transition, and the application of RNA interference targeting linc‐ ITGB 1 might be a potential form of gallbladder cancer treatment in advanced cases.