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Role of the Copper( II ) Complex Cu[15]pyN 5 in Intracellular ROS and Breast Cancer Cell Motility and Invasion
Author(s) -
Fernandes Ana S.,
Flórido Ana,
Saraiva Nuno,
Cerqueira Sara,
Ramalhete Sérgio,
Cipriano Madalena,
Cabral Maria Fátima,
Miranda Joana P.,
Castro Matilde,
Costa Judite,
Oliveira Nuno G.
Publication year - 2015
Publication title -
chemical biology and drug design
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.59
H-Index - 77
eISSN - 1747-0285
pISSN - 1747-0277
DOI - 10.1111/cbdd.12521
Subject(s) - intracellular , doxorubicin , motility , cancer research , breast cancer , cancer cell , cell migration , apoptosis , mcf 7 , cell , chemistry , cell growth , cell culture , cancer , chemotherapy , biology , microbiology and biotechnology , medicine , biochemistry , human breast , genetics
Multiple mechanisms related to metastases undergo redox regulation. Cu[15]pyN 5 is a redox‐active copper( II ) complex previously studied as a chemotherapy sensitizer in mammary cells. The effects of a cotreatment with Cu[15]pyN 5 and doxorubicin (dox) were evaluated in two human breast cancer cell lines: MCF 7 (low aggressiveness) and MDA ‐ MB ‐231 (highly aggressive). Cu[15]pyN 5 decreased MCF 7‐directed cell migration. In addition, a cotreatment with dox and Cu[15]pyN 5 reduced the proteolytic invasion of MDA ‐ MB ‐231 cells. Cell detachment was not affected by exposure to these agents. Cu[15]pyN 5 and dox significantly increased intracellular ROS in both cell lines. This increase could be at least partially due to H 2 O 2 accumulation. The combination of Cu[15]pyN 5 with dox may be beneficial in breast cancer treatment as it could help reduce cancer cell migration and invasion. Moreover, the ligand [15]pyN 5 has a high affinity for copper( II ) and displays potential anti‐angiogenic properties. Overall, we present a potential drug that might arrest the progression of breast cancer by different and complementary mechanisms.