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A Fluorescent Probe for Imaging p53– MDM 2 Protein–Protein Interaction
Author(s) -
Liu Zhenzhen,
Miao Zhenyuan,
Li Jin,
Fang Kun,
Zhuang Chunlin,
Du Lupei,
Sheng Chunquan,
Li Minyong
Publication year - 2015
Publication title -
chemical biology and drug design
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.59
H-Index - 77
eISSN - 1747-0285
pISSN - 1747-0277
DOI - 10.1111/cbdd.12434
Subject(s) - fluorescence , chemistry , biophysics , protein–protein interaction , in vitro , fluorescent protein , bimolecular fluorescence complementation , p53 protein , molecular probe , green fluorescent protein , small molecule , biochemistry , microbiology and biotechnology , biology , dna , gene , physics , quantum mechanics
In this article, we describe a no‐wash small‐molecule fluorescent probe for detecting and imaging p53– MDM 2 protein–protein interaction based on an environment‐sensitive fluorescent turn‐on mechanism. After extensive biological examination, this probe L1 exhibited practical activity and selectivity in vitro and in cellulo.