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Down‐Regulation of Asparagine Synthetase Induces Cell Cycle Arrest and Inhibits Cell Proliferation of Breast Cancer
Author(s) -
Yang Hongjian,
He Xiangming,
Zheng Yabing,
Feng Weiliang,
Xia Xianghou,
Yu Xingfei,
Lin Zhiqiang
Publication year - 2014
Publication title -
chemical biology and drug design
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.59
H-Index - 77
eISSN - 1747-0285
pISSN - 1747-0277
DOI - 10.1111/cbdd.12348
Subject(s) - breast cancer , cancer research , cell growth , carcinogenesis , cell cycle , asparagine synthetase , cancer , cancer cell , gene silencing , biology , medicine , asparagine , enzyme , biochemistry , gene
Asparagine synthetase ( ASNS ) is deemed to be a promising therapeutic target for the treatment of several cancers, but its functional role in human breast cancer is still unknown. In this study, we employed RNA interference as an efficient tool to silence endogenous ASNS expression in breast cancer cell lines. The relationship between ASNS expression and breast cancer cell growth was investigated, and the therapeutic value of ASNS in breast cancer was further evaluated. Depletion of ASNS remarkably inhibited the proliferation and colony formation capacity of breast cancer cells and arrested cell cycle in the S phase. Our findings suggest that ASNS may contribute to breast cancer tumorigenesis and could be a potential therapeutic target in human breast cancer.