Premium
In vitro Evaluation of Doxorubicin‐Incorporated Magnetic Albumin Nanospheres
Author(s) -
Zeybek Ayça,
ŞanlıMohamed Gülşah,
Ak Güliz,
Yılmaz Habibe,
Şanlıer Şenay H.
Publication year - 2014
Publication title -
chemical biology and drug design
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.59
H-Index - 77
eISSN - 1747-0285
pISSN - 1747-0277
DOI - 10.1111/cbdd.12300
Subject(s) - doxorubicin , nanocarriers , drug delivery , flow cytometry , chemistry , mtt assay , targeted drug delivery , bovine serum albumin , cytotoxicity , albumin , nanocapsules , biophysics , in vitro , pharmacology , materials science , microbiology and biotechnology , nanotechnology , biochemistry , nanoparticle , chemotherapy , medicine , biology , surgery , organic chemistry
Magnetic albumin nanospheres that incorporate doxorubicin (M‐ DOX ‐ BSA ‐ NP s) were prepared previously by our research group to develop magnetically responsive drug carrier system. This nanocarrier was synthesized as a drug delivery system for targeted chemotherapy. In this work, cytotoxic effects of doxorubicin ( DOX )‐loaded/unloaded or magnetic/non‐magnetic nanoparticles and free DOX against PC ‐3 cells and A549 cells were determined with the MTT test and the results were compared with each other. DOX ‐loaded magnetic albumin nanospheres (M‐ DOX ‐ BSA ‐ NP s) were found more cytotoxic than other formulations. The quantitative data obtained from flow cytometry analysis further verified the higher targeting and killing ability of M‐ DOX ‐ BSA ‐ NP s than free DOX on both of the cancer cell lines. Additionally, the results of cell cycle analysis have showed that M‐ DOX ‐ BSA ‐ NP s affected G1 and G2 phases. Finally, cell images were obtained using spin‐disk confocal microscopy, and cellular uptake of M‐ DOX ‐ BSA ‐ NP s was visualized. The findings of this study suggest that M‐ DOX ‐ BSA ‐ NP s represent a potential doxorubicin delivery system for targeted drug transport into prostate and lung cancer cells.