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Preparation, Optimization and Characterization of Bovine Lactoferrin‐loaded Liposomes and Solid Lipid Particles Modified by Hydrophilic Polymers Using Factorial Design
Author(s) -
Yao Xudong,
Bunt Craig,
Cornish Jillian,
Quek SiewYoung,
Wen Jingyuan
Publication year - 2014
Publication title -
chemical biology and drug design
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.59
H-Index - 77
eISSN - 1747-0285
pISSN - 1747-0277
DOI - 10.1111/cbdd.12269
Subject(s) - differential scanning calorimetry , chitosan , liposome , chromatography , bioavailability , polymer , fourier transform infrared spectroscopy , particle size , pectin , controlled release , materials science , chemistry , chemical engineering , organic chemistry , biochemistry , nanotechnology , biology , physics , engineering , thermodynamics , bioinformatics
Bioadhesive liposomes and solid lipid particles (SLPs) modified by pectin and chitosan for oral administration of bovine lactoferrin ( bL f) were prepared using a 2 4 full‐factorial design to identify the key formulation variables influencing particle size and drug entrapment efficiency (EE). Netlike structures of the polymer–particle mixture consisting of a polymeric network in which multiple particles were imbedded were observed by scanning electron microscopy (SEM). Chemical stability of bL f after encapsulation into pectin‐ and chitosan‐modified liposomes and SLPs was confirmed by Fourier transform infrared spectra (FTIR). Bovine lactoferrin was located within phospholipid bilayer, whereas in SLPs bL f was within the matrix. The crystalline nature of bL f after encapsulation was investigated by differential scanning calorimetry (DSC) of drug‐loaded particles, indicating amorphous dispersion of bL f in the polymer–lipid matrix of pectin‐ and chitosan‐modified liposomes and SLPs. In vivo pharmacokinetic investigation of bL f in pectin‐ and chitosan‐modified liposomes and SLPs showed prolonged mean residence time (MRT) of bL f in rat blood and increased the relative bioavailability (F bio %) by 1.95‐ to 2.69‐fold compared with free bL f. The developed carrier systems are considered to be promising vehicles for oral delivery.