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Unsymmetrical Oxovanadium Complexes Derived from Salicylaldehyde and Phenanthroline: Synthesis, DNA Interactions, and Antitumor Activities
Author(s) -
Liao Xiangwen,
Pan Weijian,
He Rongwei,
Guo Haiwei,
Ying Peng,
Lu Jiazheng
Publication year - 2014
Publication title -
chemical biology and drug design
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.59
H-Index - 77
eISSN - 1747-0285
pISSN - 1747-0277
DOI - 10.1111/cbdd.12248
Subject(s) - salicylaldehyde , apoptosis , chemistry , anthranilic acid , dna , phenanthroline , annexin , cell cycle , stereochemistry , microbiology and biotechnology , cell cycle checkpoint , cell culture , cytotoxic t cell , biochemistry , biology , crystallography , in vitro , schiff base , genetics
Two unsymmetrical oxovanadium complexes incorporating salicylaldehyde derivate and phenanthroline [ VO ( DESAA )(phen)] ( 1 ), ( DESAA = 4‐(diethylamino)salicylaldehyde anthranilic acid, phen = phenanthroline) and [ VO ( CLSAA )(phen)] ( 2 ), ( CLSAA = 5‐chlorosalicylaldehyde anthranilic acid)] have been synthesized and characterized. The interactions of the complexes with CT ‐ DNA were studied using different techniques. Complexes 1 and 2 interact with CT ‐ DNA by intercalative modes and can efficiently cleave pBR 322 DNA after light irradiation. The two complexes showed high cytotoxic activities against myeloma cell ( A g8.653) and gliomas cell ( U 251) lines. Interestingly, complex 1 exhibited greater antitumor efficiency, larger binding affinity with CT ‐ DNA , and better cleaving ability than those of complex 2 . In addition, their antitumor mechanism has been analyzed by using cell cycle analysis, apoptosis, and Annexin V ‐ FITC / PI assay. The results showed that complex 1 can cause G 2/ M ‐phase arrest of the cell cycle, exhibit a significantly induced apoptosis in A g8.653 cells, and display typical morphological apoptotic characteristics. These complexes induced proliferative suppression of A g8.653 cells via the induction of apoptosis.