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Physicochemical and Biological Characterization of P ep‐1/Elastin Complexes
Author(s) -
Ahmad Nasrollahi Saman,
Taghibiglou Changiz,
Fouladdel Shamileh,
Dinarvand Rasoul,
Moosavi Movahedi Ali A.,
Azizi Ebrahim,
Farboud Effat S.
Publication year - 2013
Publication title -
chemical biology and drug design
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.59
H-Index - 77
eISSN - 1747-0285
pISSN - 1747-0277
DOI - 10.1111/cbdd.12150
Subject(s) - cell penetrating peptide , circular dichroism , biophysics , elastin , chemistry , peptide , transdermal , peptidomimetic , transduction (biophysics) , cell , biochemistry , biology , genetics , pharmacology
Transdermal drug delivery of proteins is challenging because the skin acts as a natural and protective barrier. Several techniques including using the cell‐penetrating peptides have been studied to increase the penetration of therapeutic proteins into and through the skin. Cell‐penetrating peptides facilitate and improve the transduction of large and hydrophilic cargo molecules through plasma membrane. We have recently reported an efficient skin delivery of elastin protein in complex with a cell‐penetrating peptide called P ep‐1. As the biophysical characteristics of cell‐penetrating peptide/protein complexes have been linked with their biological responses, in this study, we investigated biophysical properties of P ep‐1/elastin complexes (ratio 10:1) stored in three temperatures (−20 °C, 4 °C and 25 °C) by photon correlation spectroscopy, circular dichroism and isothermal denaturation. We also evaluated the ability of transduction of this complex into cells and skin tissue using both fluorescence microscopy and K odak In‐Vivo FX Pro Imaging System.