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Identification of Peptides with ELAV ‐like mRNA ‐Stabilizing Effect: An Integrated In Vitro / In Silico Approach
Author(s) -
Amadio Marialaura,
Pascale Alessia,
Govoni Stefano,
Laurini Erik,
Pricl Sabrina,
Gaggeri Raffaella,
Rossi Daniela,
Collina Simona
Publication year - 2013
Publication title -
chemical biology and drug design
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.59
H-Index - 77
eISSN - 1747-0285
pISSN - 1747-0277
DOI - 10.1111/cbdd.12117
Subject(s) - in silico , messenger rna , in vitro , peptide , chemistry , untranslated region , rna , biology , biochemistry , microbiology and biotechnology , gene
Embryonic lethal abnormal vision ( ELAV ) proteins are RNA ‐binding proteins that bind specific adenine and uridine‐rich elements mainly located in the 3′‐untranslated region of target mRNA s, preventing their otherwise rapid degradation and thus increasing gene expression. Starting from our previous discovery and applying an integrated in vitro/in silico approach, herein we report a deeper understanding of the mRNA ‐stabilizing activity of four peptides derived from the ELAV proteins structure. The stabilizing effect on the VEGF transcript ( mRNA VEGF ) exerted by each peptide, tested individually, was initially evaluated, and no effects were evidenced. Hence, the biological effects of all peptides couples were investigated. Interestingly, in accordance with preliminary molecular dynamics results, only one of all possible peptide couples resulted highly effective in stabilizing mRNA VEGF . These two peptides were thus identified as valuable starting point for designing small molecules with ELAV ‐mimicking properties.