Open AccessIdentification of cancer driver genes using Sleeping Beauty transposon mutagenesis
Author(s) -
Takeda Haruna,
Jenkins Nancy A.,
Copeland Neal G.
Publication year - 2021
Publication title -
cancer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 1347-9032
DOI - 10.1111/cas.14901
Subject(s) - transposon mutagenesis , sleeping beauty transposon system , gene , mutagenesis , biology , transposable element , cancer , genetic screen , genetics , genome , identification (biology) , computational biology , forward genetics , mutation , phenotype , botany
Cancer genome sequencing studies have identified driver genes for a variety of different cancers and helped to understand the genetic landscape of human cancer. It is still challenging, however, to identify cancer driver genes with confidence simply from genetic data alone. In vivo forward genetic screens using Sleeping Beauty ( SB ) transposon mutagenesis provides another powerful genetic tool for identifying candidate cancer driver genes in wild‐type and sensitized mouse tumors. By comparing cancer driver genes identified in human and mouse tumors, cancer driver genes can be identified with additional confidence based upon comparative oncogenomics. This review describes how SB mutagenesis works in mice and focuses on studies that have identified cancer driver genes in the mouse gastrointestinal tract.