Open AccessDiscovery of inner centromere protein‐derived small peptides for cancer imaging and treatment targeting survivin
Author(s) -
Fuchigami Takeshi,
Ishikawa Natsumi,
Nozaki Iori,
Miyanari Yusuke,
Yoshida Sakura,
Yamauchi Motohiro,
Soejima Ayumi,
Haratake Mamoru,
Nakayama Morio
Publication year - 2020
Publication title -
cancer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 1347-9032
DOI - 10.1111/cas.14330
Subject(s) - survivin , centromere , cancer , biology , cancer research , computational biology , medicine , genetics , gene , chromosome
Survivin belongs to the inhibitor of apoptosis protein family, which is consistently overexpressed in most cancer cells but rarely expressed in normal adult tissues. Therefore, the detection and inhibition of survivin are regarded as attractive strategies for cancer‐specific treatment. In this study, we designed and synthesized 7‐19 residues of inner centromere protein (INCENP)‐derived small peptides (INC peptides) as novel survivin‐targeting agents. The INC peptides showed binding affinity for the human survivin protein ( K d = 91.4‐255 nmol L −1 ); INC 16‐22 , which contains residues 16‐22 of INCENP, showed the highest affinity (91.4 nmol L −1 ). Confocal fluorescence imaging showed consistent colocalization of FITC‐INC 16‐22 and survivin in cell lines. Nona‐arginine‐linked INC 16‐22 (r9‐INC 16‐22 ) rendered INC 16‐22 cells penetrable and strongly inhibited cell growth of MIA PaCa‐2 cells (52% inhibition at 1.0 µmol L −1 ) and MDA‐MB‐231 cells (60% inhibition at 10 µmol L −1 ) as determined by MTT assays. The exposure of MIA PaCa‐2 cells to 40 µmol L −1 r9‐INC 16‐22 apparently reduced the intracellular protein expression levels of survivin. However, cleaved caspase‐3 was significantly increased in cells treated with r9‐INC 16‐22 , even at 10 µmol L −1 , compared to untreated cells. Flow cytometry revealed that r9‐INC 16‐22 strongly induced apoptosis in MIA PaCa‐2 cells. These results indicate that the cytotoxic effects of r9‐INC 16‐22 could be mediated mainly through the disruption of survivin‐dependent antiapoptotic functions and partly because of the direct degradation of the survivin protein. Our findings suggest that INC peptides can act as useful scaffolds for novel cancer imaging and anticancer agents.