Open AccessMiR‐1285‐5p/ TMEM194A axis affects cell proliferation in breast cancer
Author(s) -
HironakaMitsuhashi Ai,
Otsuka Kurataka,
Gailhouste Luc,
Sanchez Calle Anna,
Kumazaki Minami,
Yamamoto Yusuke,
Fujiwara Yasuhiro,
Ochiya Takahiro
Publication year - 2020
Publication title -
cancer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 1347-9032
DOI - 10.1111/cas.14287
Subject(s) - breast cancer , microrna , gene knockdown , cancer research , suppressor , cancer , centrosome , cell growth , medicine , transmembrane protein , oncology , biology , gene , cell cycle , receptor , genetics
The onset of breast cancer among young patients is a major issue in cancer etiology. Our previous study has shown that poor prognosis in young women with breast cancer is associated with lower expression of the microRNA miR‐1285‐5p. In this study, we showed that the expression of miR‐1285‐5p is lower in tumor tissues than in normal tissues. Accumulating evidence suggests that miR‐1285‐5p plays critical roles in various types of cancers. However, the functional role of miR‐1285‐5p in breast cancer remains to be elucidated. Here, we showed the tumor‐suppressive role of miR‐1285‐5p and detailed its mechanism of action in breast cancer. Overexpression of miR‐1285‐5p significantly inhibited cell proliferation in breast cancer cells regardless of the tumor subtype. Among the target genes of miR‐1285‐5p, we found that transmembrane protein 194A ( TMEM194A ) was directly regulated by miR‐1285‐5p. Notably, separation of centrosomes from the nuclear envelope was observed upon knockdown of TMEM194A or overexpression of miR‐1285‐5p. In conclusion, our findings show that miR‐1285‐5p is a tumor suppressor via TMEM194A inhibition in breast cancer.