Open AccessNeural precursor cell expressed, developmentally downregulated 8 promotes tumor progression and predicts poor prognosis of patients with bladder cancer
Author(s) -
Tian DaWei,
Wu ZhouLiang,
Jiang LiMing,
Gao Jie,
Wu ChangLi,
Hu HaiLong
Publication year - 2019
Publication title -
cancer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 1347-9032
DOI - 10.1111/cas.13865
Subject(s) - bladder cancer , immunohistochemistry , cancer research , metastasis , biology , cell cycle , cell growth , tumor progression , cancer , in vivo , apoptosis , cell migration , western blot , cell , pathology , medicine , immunology , gene , genetics
Neddylation has been researched in many different human carcinomas. However, the roles of neural precursor cell expressed, developmentally downregulated 8 ( NEDD 8) in bladder cancer are still unknown. Our study was the first study which systematically investigated the possible functions of NEDD 8 in bladder cancer ( BC ) progression. We carried out immunohistochemistry to explore associations between the expression of NEDD 8 in tumor tissues and clinical outcomes of patients. RT ‐ qPCR and western blot were used to detect the expressional levels of genes. The biological abilities of cell proliferation, migration and invasion were researched by in vitro and in vivo experiments. Results were as follows: Data from The Cancer Genome Atlas ( TCGA ) database showed that NEDD 8 was overexpressed in BC tissues and was associated with poor patient survival. Results of immunohistochemistry found that NEDD 8 was significantly associated with poor clinical outcomes of BC patients. Suppression of NEDD 8 could inhibit the proliferation, migration and invasion of tumor cells. Knocking down NEDD 8 could induce apoptosis and G2 phase arrest of cell cycle progression. In vivo, suppression of NEDD 8 restricted growth and metastasis of tumors in mice. In conclusion, NEDD 8 has important roles in regulating the progression of BC cells and was associated with poor prognosis of patients; hence, it may become a potential therapeutic target of BC .