Open AccessNext generation sequencing‐based gene panel tests for the management of solid tumors
Author(s) -
Nagahashi Masayuki,
Shimada Yoshifumi,
Ichikawa Hiroshi,
Kameyama Hitoshi,
Takabe Kazuaki,
Okuda Shujiro,
Wakai Toshifumi
Publication year - 2019
Publication title -
cancer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 1347-9032
DOI - 10.1111/cas.13837
Subject(s) - dna sequencing , microsatellite instability , computational biology , genetic testing , genomics , gene , genome , genetics , biology , bioinformatics , medicine , microsatellite , allele
Next generation sequencing (NGS) has been an invaluable tool to put genomic sequencing into clinical practice. The incorporation of clinically relevant target sequences into NGS‐based gene panel tests has generated practical diagnostic tools that enable individualized cancer‐patient care. The clinical utility of gene panel testing includes investigation of the genetic basis for an individual's response to therapy, such as signaling pathways associated with a response to specific therapies, microsatellite instability and a hypermutated phenotype, and deficiency in the DNA double‐strand break repair pathway. In this review, we describe the concept of precision cancer medicine using target sequences in gene panel tests as well as the importance of the control of sample quality in routine NGS‐based genomic testing. We describe geographic and ethnic differences in cancer genomes, and discuss issues that need to be addressed in the future based on our experiences in Japan.