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The  PIM 1 protein is an important regulator of cell proliferation, the cell cycle, apoptosis, and metabolism in various human cancers. Micro RNA s (mi RNA s) are powerful post‐transcriptional gene regulators that function through translational repression or transcript destabilization. Therefore, we aimed to identify whether a close relationship exists between  PIM 1 and mi RNA s.  PIM 1 protein levels and  mRNA  levels were significantly upregulated in astrocytoma tissues, indicating the oncogenic role of  PIM 1 in astrocytoma. Further bioinformatics analysis indicated that miR‐124‐3p targeted the 3′‐ UTR  of  PIM 1. We also observed an inverse correlation between the miR‐124‐3p levels and  PIM 1 protein or  mRNA  levels in astrocytoma samples. Next, we experimentally confirmed that miR‐124‐3p directly recognizes the 3′‐ UTR  of the  PIM 1 transcript and regulates  PIM 1 expression at both the protein and  mRNA  levels. Furthermore, we examined the biological consequences of miR‐124‐3p targeting  PIM 1  in vitro . We showed that the repression of  PIM 1 in astrocytoma cancer cells by miR‐124‐3p suppressed proliferation, invasion, and aerobic glycolysis and promoted apoptosis. We observed that the restoration or inhibition of  PIM 1 activity resulted in effects that were similar to those induced by miR‐124‐3p inhibitors or mimics in cancer cells. Finally, overexpression of  PIM 1 rescued the inhibitory effects of miR‐124‐3p. In summary, these findings aid in understanding the tumor‐suppressive role of miR‐124‐3p in astrocytoma pathogenesis through the inhibition of  PIM 1 translation.

MicroRNA‐124‐3p regulates cell proliferation, invasion, apoptosis, and bioenergetics by targeting PIM1 in astrocytoma