Open AccessRetracted: Micro RNA ‐34a suppresses the breast cancer stem cell‐like characteristics by downregulating Notch1 pathway
Author(s) -
Kang Le,
Mao Jun,
Tao Yajun,
Song Bo,
Ma Wei,
Lu Ying,
Zhao Lijing,
Li Jiazhi,
Yang Baoxue,
Li Lianhong
Publication year - 2015
Publication title -
cancer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 1347-9032
DOI - 10.1111/cas.12656
Subject(s) - microrna , cancer research , breast cancer , stem cell , cancer stem cell , notch signaling pathway , cell growth , paclitaxel , metastasis , cancer , cell , biology , signal transduction , medicine , microbiology and biotechnology , gene , biochemistry
Micro RNA s play pivotal roles in cancer stem cell regulation. Previous studies have shown that micro RNA ‐34a (miR‐34a) is downregulated in human breast cancer. However, it is unknown whether and how miR‐34a regulates breast cancer stem cells. Notch signaling is one of the most important pathways in stem cell maintenance and function. In this study, we verified that miR‐34a directly and functionally targeted Notch1 in MCF ‐7 cells. We reported that miR‐34a negatively regulated cell proliferation, migration, and invasion and breast cancer stem cell propagation by downregulating Notch1. The expression of miR‐34a was negatively correlated with tumor stages, metastasis, and Notch1 expression in breast cancer tissues. Furthermore, overexpression of miR‐34a increased chemosensitivity of breast cancer cells to paclitaxel ( PTX ) by downregulating the Notch1 pathway. Mammosphere formation and expression of the stemness factor ALDH 1 were also reduced in the cells treated with miR‐34a and PTX compared to those treated with PTX alone. Taken together, our results indicate that miR‐34a inhibited breast cancer stemness and increased the chemosensitivity to PTX partially by downregulating the Notch1 pathway, suggesting that miR‐34a/Notch1 play an important role in regulating breast cancer stem cells. Thus miR‐34a is a potential target for prevention and therapy of breast cancer.