Open AccessTelomelysin shows potent antitumor activity through apoptotic and non‐apoptotic cell death in soft tissue sarcoma cells
Author(s) -
Li GuiDong,
Kawashima Hiroyuki,
Ogose Akira,
Ariizumi Takashi,
Hotta Tetsuo,
Kuwano Ryozo,
Urata Yasuo,
Fujiwara Toshiyoshi,
Endo Naoto
Publication year - 2013
Publication title -
cancer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 1347-9032
DOI - 10.1111/cas.12208
Subject(s) - apoptosis , apoptotic cell death , soft tissue sarcoma , cancer research , programmed cell death , sarcoma , soft tissue , cell , medicine , pathology , biology , biochemistry
This study investigated the pathway underlying the antitumor activity of telomelysin, a telomerase‐dependent, replication‐selective oncolytic adenovirus, in soft tissue sarcoma cells. Treatment with telomelysin alone resulted in simultaneous induction of apoptosis and autophagy, whereas cotreatment with telomelysin and 3‐methyladenine significantly reduced cell viability and increased apoptosis and the cellular ATP level compared to treatment with telomelysin alone, indicating that telomelysin‐mediated autophagy is a death‐protective but not death‐promoting process. Cotreatment with Z‐Val‐Ala‐Asp‐ CH 2F significantly increased cellular ATP depletion compared to telomelysin‐alone treatment while inhibiting telomelysin‐induced apoptosis and having no significant effect on cell viability, indicating that it promotes transition from apoptotic to necrotic cell death.