Premium
Three‐dimensional cell culture model utilization in cancer stem cell research
Author(s) -
Bielecka Zofia F.,
MaliszewskaOlejniczak Kamila,
Safir Ilan J.,
Szczylik Cezary,
Czarnecka Anna M.
Publication year - 2017
Publication title -
biological reviews
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.993
H-Index - 165
eISSN - 1469-185X
pISSN - 1464-7931
DOI - 10.1111/brv.12293
Subject(s) - cancer stem cell , 3d cell culture , stem cell , cell culture , cancer , biology , microbiology and biotechnology , cancer research , genetics
Three‐dimensional (3D) cell culture models are becoming increasingly popular in contemporary cancer research and drug resistance studies. Recently, scientists have begun incorporating cancer stem cells (CSCs) into 3D models and modifying culture components in order to mimic in vivo conditions better. Currently, the global cell culture market is primarily focused on either 3D cancer cell cultures or stem cell cultures, with less focus on CSCs. This is evident in the low product availability officially indicated for 3D CSC model research. This review discusses the currently available commercial products for CSC 3D culture model research. Additionally, we discuss different culture media and components that result in higher levels of stem cell subpopulations while better recreating the tumor microenvironment. In summary, although progress has been made applying 3D technology to CSC research, this technology could be further utilized and a greater number of 3D kits dedicated specifically to CSCs should be implemented.