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Multiple role of 3‐mercaptopyruvate sulfurtransferase: antioxidative function, H 2 S and polysulfide production and possible SO x production
Author(s) -
Nagahara Noriyuki
Publication year - 2018
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/bph.14100
Subject(s) - sulfurtransferase , polysulfide , redox , chemistry , cysteine , sulfur , reducing agent , dimer , thioredoxin , rhodanese , active site , sulfide , half reaction , hydrogen sulfide , catalysis , enzyme , photochemistry , biochemistry , inorganic chemistry , organic chemistry , electrode , electrolyte
Rat 3‐mercaptopyruvate sulfurtransferase (MPST) is a 32 808 Da simple protein. Cys 247 is a catalytic site, and Cys 154 and Cys 263 are on the enzyme surface. MPST is found in all tissues, particularly in the kidneys, although the localization of its activity differs in each tissue. In this review, four functions of MPST are reviewed: (i) antioxidative function: Cys 247 is redox‐sensitive and serves as a redox‐sensing switch. It is oxidized to cysteine sulfenate, which has a low redox potential, upon which the enzyme is inactivated. Then, reduced thioredoxin (Trx) with a reducing system (Trx reductase and NADPH) reduces the sulfenate to restore activity; meanwhile, Cys 154 and Cys 263 form an intermolecular disulfide bond, which serves as another redox‐sensing switch. Consequently, Trx specifically cleaves the intermolecular disulfide bond by converting it from the inactive form (dimer) to the active form (monomer). (ii) Hydrogen sulfide and polysulfide production: hydrogen sulfide is produced via reduction of the persulfurated sulfur‐acceptor substrate by reduced Trx or Trx with a reducing system; as an alternative process, stable polysulfurated or persulfurated Cys 247 as a reaction intermediate is reduced by Trx with a reducing system to release hydrogen sulfide and polysulfides. (iii) Possible sulfur oxide production: sulfur oxides (SO, SO 2 and SO 3 ) can be produced in the redox cycle of sulfane sulfur formed at the catalytic site Cys 247 (Cys–SO − , Cys–SO 2− and Cys–SO 3− ) as reaction intermediates and released by reduced Trx or Trx with a reducing system. (iv) Possible anxiolytic‐like effects: MPST‐knockout mice exhibited anxiolytic‐like effects.