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Chemoresistant lung cancer stem cells display high DNA repair capability to remove cisplatin‐induced DNA damage
Author(s) -
Yu WaiKin,
Wang Zhigang,
Fong ChiChun,
Liu Dandan,
Yip TakChun,
Au SiuKie,
Zhu Guangyu,
Yang Mengsu
Publication year - 2017
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/bph.13690
Subject(s) - cisplatin , dna repair , dna damage , biology , dna , cancer research , stem cell , transcription factor , microbiology and biotechnology , genetics , gene , chemotherapy
Background and Purpose The persistence of lung cancer stem cells (LCSCs) has been proposed to be the main factor responsible for the recurrence of lung cancer as they are highly resistant to conventional chemotherapy. However, the underlying mechanisms are still unclear. Experimental Approach We examined the cellular response of a human LCSC line to treatment with cisplatin, a DNA‐damaging anticancer drug that is used extensively in the clinic. We compared the response to cisplatin of LCSCs and differentiated LCSCs (dLCSCs) by determining the viability of these cells, and their ability to accumulate cisplatin and to implement genomic and transcription‐coupled DNA repair. We also investigated the transcription profiles of genes related to drug transport and DNA repair. Key Results LCSCs were found to be more stem‐like, and more resistant to cisplatin‐induced cytotoxicity than dLCSCs, confirming their drug resistance properties. LCSCs accumulated less cisplatin intracellularly than dLCSCs and showed less DNA damage, potentially due to their ability to down‐regulate AQP2 and CTR1. The results of the transcription‐coupled repair of cisplatin‐DNA cross‐links indicated a higher level of repair of DNA damage in LCSCs than in dLCSCs. In addition, LCSCs showed a greater ability to repair cisplatin‐DNA interstrand cross‐links than dLCSCs; this involved the activation of various DNA repair pathways. Conclusions and Implications Our results further clarify the mechanism of cisplatin resistance in LCSCs in terms of reduced cisplatin uptake and enhanced ability to implement DNA repairs. These findings may aid in the design of the next‐generation of platinum‐based anticancer drugs.