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Alterations in perivascular innervation function in mesenteric arteries from offspring of diabetic rats
Author(s) -
Queiroz D B,
Sastre E,
Caracuel L,
Callejo M,
Xavier F E,
BlancoRivero J,
Balfagón G
Publication year - 2015
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/bph.13244
Subject(s) - endocrinology , medicine , vasomotor , vasoconstriction , mesenteric arteries , phentolamine , offspring , blood pressure , vasodilation , sympathetic nervous system , stimulation , artery , biology , pregnancy , genetics
Background and Purpose We have reported that exposure to a diabetic intrauterine environment during pregnancy increases blood pressure in adult offspring, but the mechanisms involved are not completely understood. This study was designed to analyse a possible role of perivascular sympathetic and nitrergic innervation in the superior mesenteric artery ( SMA ) in this effect. Experimental Approach Diabetes was induced in pregnant Wistar rats by a single injection of streptozotocin. Endothelium‐denuded vascular rings from the offspring of control ( O ‐ CR ) and diabetic rats ( O ‐ DR ) were used. Vasomotor responses to electrical field stimulation ( EFS ), NA and the NO donor DEA‐NO were studied. The expressions of neuronal NOS (n NOS ) and phospho‐nNOS ( P ‐n NOS ) and release of NA , ATP and NO were determined. Sympathetic and nitrergic nerve densities were analysed by immunofluorescence. Key Results Blood pressure was higher in O ‐ DR animals. EFS ‐induced vasoconstriction was greater in O ‐ DR animals. This response was decreased by phentolamine more in O ‐ DR animals than their controls. L‐NAME increased EFS ‐induced vasoconstriction more strongly in O ‐ DR than in O ‐ CR segments. Vasomotor responses to NA or DEA‐NO were not modified. NA , ATP and NO release was increased in segments from O ‐ DR . n NOS expression was not modified, whereas P ‐n NOS expression was increased in O ‐ DR . Sympathetic and nitrergic nerve densities were similar in both experimental groups. Conclusions and Implications The activity of sympathetic and nitrergic innervation is increased in SMA from O ‐ DR animals. The net effect is an increase in EFS ‐induced contractions in these animals. These effects may contribute to the increased blood pressure observed in the offspring of diabetic rats.