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Localization of sunitinib, its metabolites and its target receptors in tumour‐bearing mice: a MALDI ‐MS imaging study
Author(s) -
Torok S,
Vegvari A,
Rezeli M,
Fehniger T E,
Tovari J,
Paku S,
Laszlo V,
Hegedus B,
Rozsas A,
Dome B,
MarkoVarga G
Publication year - 2015
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/bph.12990
Subject(s) - sunitinib , receptor , chemistry , cancer research , pathology , pharmacology , biology , medicine , cancer , biochemistry
Background and Purpose The clinical effects of anti‐angiogenic agents remain controversial. Therefore, elucidating the pharmacological properties of these compounds is a pivotal issue. Experimental Approach The effects of treatment with sunitinib on tumour and normal tissues of mice bearing C‐26 adenocarcinoma cells were analysed by matrix‐assisted laser desorption ionization MS imaging ( MALDI‐MSI ). Expression of the key targets of sunitinib – angiogenic receptors – was studied by immunofluorescent labelling. Key Results MALDI‐MS assays showed that sunitinib and its fragment ions were present throughout tumour and normal tissues. Major metabolites were identified in blood and solid tissues, while minor drug metabolites were detectable only in blood. Tumour growth and intratumour VEGF receptor‐2 expressions were significantly reduced in sunitinib‐treated mice, while the expression of the other targeted receptors, PDGF receptor ‐ α or ‐ β and fibroblast growth factor receptor‐1, remained unaffected. Within tumour tissue, the close proximity of sunitinib metabolites to the precursor ion suggested in situ metabolism of the administered drug. There were intratumour areas where the signal intensity of sunitinib correlated with expression of VEGF receptor‐2. Conclusions and Implications This is the first study that demonstrates MALDI‐MSI is a versatile platform to study the intratumour localization of an unlabelled anti‐angiogenic drug. The combination of MALDI‐MSI and immunofluorescence analysis can provide further insights into the molecular interaction of drug compounds and their targets within tumour tissue.