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Protective effects of n‐6 fatty acids‐enriched diet on intestinal ischaemia/reperfusion injury involve lipoxin A 4 and its receptor
Author(s) -
Gobbetti T,
Ducheix S,
Faouder P,
Perez T,
Riols F,
Boue J,
BertrandMichel J,
Dubourdeau M,
Guillou H,
Perretti M,
Vergnolle N,
Cenac N
Publication year - 2015
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/bph.12957
Subject(s) - polyunsaturated fatty acid , inflammation , lipoxin , receptor , endocrinology , medicine , ischemia , intestinal mucosa , chemistry , endogeny , biology , biochemistry , fatty acid
Background and Purpose Long‐term intake of dietary fatty acids is known to predispose to chronic inflammation, but their effects on acute intestinal ischaemia/reperfusion ( I/R ) injury is unknown. The aim of this study was to determine the consequences of a diet rich in n‐3 or n‐6 polyunsaturated fatty acids ( PUFA ) on intestinal I/R ‐induced damage. Experimental Approach Mice were fed three different isocaloric diets: a balanced diet used as a control and two different PUFA ‐enriched diets, providing either high levels of n‐3 or of n‐6 PUFA. Intestinal injury was evaluated after intestinal I/R . PUFA metabolites were quantitated in intestinal tissues by LC‐MS/MS . Key Results In control diet‐fed mice, intestinal I/R caused inflammation and increased COX and lipoxygenase‐derived metabolites compared with sham‐operated animals. Lipoxin A 4 ( LxA 4 ) was significantly and selectively increased after ischaemia. Animals fed a high n‐3 diet did not display a different inflammatory profile following intestinal I/R compared with control diet‐fed animals. In contrast, intestinal inflammation was decreased in the I/R group fed with high n‐6 diet and level of LxA 4 was increased post‐ischaemia compared with control diet‐fed mice. Blockade of the LxA 4 receptor ( Fpr2 ), prevented the anti‐inflammatory effects associated with the n‐6 rich diet. Conclusions and Implications This study indicates that high levels of dietary n‐6, but not n‐3, PUFAs provides significant protection against intestinal I/R ‐induced damage and demonstrates that the endogenous production of LxA 4 can be influenced by diet.