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Drp 1 is dispensable for apoptotic cytochrome c release in primed MCF 10 A and fibroblast cells but affects Bcl ‐2 antagonist‐induced respiratory changes
Author(s) -
Clerc P,
Ge S X,
Hwang H,
Waddell J,
Roelofs B A,
Karbowski M,
Sesaki H,
Polster B M
Publication year - 2014
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/bph.12515
Subject(s) - cytochrome c , apoptosis , biology , programmed cell death , cytochrome , microbiology and biotechnology , apoptosome , biochemistry , caspase , enzyme
Dynamin-related protein 1 (Drp1) mediates mitochondrial fission and is thought to promote Bax/Bak-induced cytochrome c release during apoptosis. Conformationally active Bax, Bak and Bax/Bak-activating BH3-only proteins, such as Bim, are restrained by anti-apoptotic Bcl-2 proteins in cells that are 'primed for death'. Inhibition of Bcl-2/Bcl-xL/Bcl-w by the antagonist ABT-737 causes rapid apoptosis of primed cells. Hence, we determined whether Drp1 is required for cytochrome c release, respiratory alterations and apoptosis of cells that are already primed for death.