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Pharmacological profiling of the TRPV3 channel in recombinant and native assays
Author(s) -
Grubisha Olivera,
Mogg Adrian J,
Sorge Jessica L,
Ball LauraJayne,
Sanger Helen,
Ruble Cara L A,
Folly Elizabeth A,
Ursu Daniel,
Broad Lisa M
Publication year - 2014
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/bph.12303
Subject(s) - recombinant dna , endogeny , high throughput screening , biology , pharmacology , agonist , receptor , biochemistry , gene
Transient receptor potential vanilloid subtype 3 (TRPV3) is implicated in nociception and certain skin conditions. As such, it is an attractive target for pharmaceutical research. Understanding of endogenous TRPV3 function and pharmacology remains elusive as selective compounds and native preparations utilizing higher throughput methodologies are lacking. In this study, we developed medium-throughput recombinant and native cellular assays to assess the detailed pharmacological profile of human, rat and mouse TRPV3 channels.