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N ‐arachidonoyl glycine suppresses Na + / Ca 2+ exchanger‐mediated Ca 2+ entry into endothelial cells and activates BK Ca channels independently of GPCR s
Author(s) -
Bondarenko Alexander I,
Drachuk Konstantin,
Panasiuk Olga,
Sagach Vadim,
Deak Andras T,
Malli Roland,
Graier Wolfgang F
Publication year - 2013
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/bph.12180
Subject(s) - hyperpolarization (physics) , chemistry , histamine , biophysics , endothelial stem cell , membrane potential , biochemistry , pharmacology , stereochemistry , biology , in vitro , nuclear magnetic resonance spectroscopy
Background and Purpose N ‐arachidonoyl glycine ( NAGly ) is a lipoamino acid with vasorelaxant properties. We aimed to explore the mechanisms of NAGly's action on unstimulated and agonist‐stimulated endothelial cells. Experimental Approach The effects of NAGly on endothelial electrical signalling were studied in combination with vascular reactivity. Key Results I n EA .hy926 cells, the sustained hyperpolarization to histamine was inhibited by the non‐selective Na + / Ca 2+ exchanger ( NCX ) inhibitor bepridil and by an inhibitor of reversed mode NCX, KB ‐R7943. In cells dialysed with Cs + ‐based Na + ‐containing solution, the outwardly rectifying current with typical characteristics of NCX was augmented following histamine exposure, further increased upon external Na + withdrawal and inhibited by bepridil. NAGly (0.3–30 μM) suppressed NCX currents in a URB597 ‐ and guanosine 5′‐O‐(2‐thiodiphosphate) ( GDP β S )‐insensitive manner, [ Ca 2+ ] i elevation evoked by Na + removal and the hyperpolarization to histamine. In rat aorta, NAGly opposed the endothelial hyperpolarization and relaxation response to ACh. In unstimulated EA .hy926 cells, NAGly potentiated the whole‐cell current attributable to large‐conductance Ca 2+ ‐activated K + ( BK Ca ) channels in a GDP β S ‐insensitive, paxilline‐sensitive manner and produced a sustained hyperpolarization. In cell‐free inside‐out patches, NAGly stimulated single BK Ca channel activity. Conclusion and Implications Our data showed that NCX is a Ca 2+ entry pathway in endothelial cells and that NAGly is a potent G ‐protein‐independent modulator of endothelial electrical signalling and has a dual effect on endothelial electrical responses. In agonist pre‐stimulated cells, NAGly opposes hyperpolarization and relaxation via inhibition of NCX ‐mediated Ca 2+ entry, while in unstimulated cells, it promotes hyperpolarization via receptor‐independent activation of BK Ca channels.