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Olesoxime protects embryonic cortical neurons from camptothecin intoxication by a mechanism distinct from BDNF
Author(s) -
Gouarné Caroline,
GiraudonPaoli Marc,
Seimandi Mathieu,
Biscarrat Clotilde,
Tardif Gwenaëlle,
Pruss Rebecca M,
Bordet Thierry
Publication year - 2013
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/bph.12094
Subject(s) - neuroprotection , neurotrophin , neurotrophic factors , mapk/erk pathway , brain derived neurotrophic factor , neurodegeneration , neuroscience , protein kinase b , caspase , pi3k/akt/mtor pathway , microbiology and biotechnology , nerve growth factor , biology , programmed cell death , embryonic stem cell , mitochondrion , apoptosis , pharmacology , signal transduction , medicine , receptor , biochemistry , disease , gene
Background and Purpose Olesoxime is a small cholesterol–oxime promoting rat embryonic motor neurons survival in the absence of trophic factors. Because olesoxime can substitute for neurotrophic factors in many situations, and to gain further understanding of its mechanism of action, we wondered if it could prevent neuronal death induced by camptothecin ( CPT ) and compared its effects with those of brain‐derived neurotrophic factor ( BDNF ). Experimental Approach E17 rat embryonic cortical neurons were treated with olesoxime, BDNF or vehicle and intoxicated with CPT . Caspase‐dependent and caspase‐independent death pathways along with pro‐survival pathways activation were explored. Key Results As previously reported for BDNF , olesoxime dose‐dependently delayed CPT ‐induced cell death. Both compounds acted downstream of p53 activation preventing cytochrome c release and caspases activation. When caspase activation was blocked, both olesoxime and BDNF provided additional neuroprotective effect, potentially through the prevention of apoptosis‐inducing factor release from mitochondria. While BDNF activates both the PI3K / A kt and the ERK pathway, olesoxime induced only a late activation of the ERK pathways, which did not seem to play a major role in its neuroprotection against CPT . Rather, our results favour preserved mitochondrial membrane integrity by olesoxime. Conclusions and Implications Albeit different, olesoxime and BDNF mechanisms for neuroprotection converge to preserve mitochondrial function. These findings emphasize the importance of targeting the mitochondria in the process of neurodegeneration. Importantly olesoxime, by mimicking neurotrophin pro‐survival activities without impacting PI3K / A kt and ERK signalling, may have greater therapeutic potential in many diseases where neurotrophins were considered as a therapeutic solution.