
Oligodendrocyte‐specific deletion of FGFR2 ameliorates MOG 35‐55 ‐induced EAE through ERK and Akt signalling
Author(s) -
Kamali Salar,
Rajendran Ranjithkumar,
Stadelmann Christine,
Karnati Srikanth,
Rajendran Vinothkumar,
GiraldoVelasquez Mario,
Berghoff Martin
Publication year - 2021
Publication title -
brain pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.986
H-Index - 132
eISSN - 1750-3639
pISSN - 1015-6305
DOI - 10.1111/bpa.12916
Subject(s) - remyelination , oligodendrocyte , myelin , multiple sclerosis , downregulation and upregulation , fibroblast growth factor , experimental autoimmune encephalomyelitis , biology , microglia , olig2 , fibroblast growth factor receptor 1 , cancer research , inflammation , immunology , receptor , endocrinology , central nervous system , biochemistry , gene
Fibroblast growth factors (FGFs) and their receptors (FGFRs) are involved in demyelinating pathologies including multiple sclerosis (MS). In our recent study, oligodendrocyte‐specific deletion of FGFR1 resulted in a milder disease course, less inflammation, reduced myelin and axon damage in EAE. The objective of this study was to elucidate the role of oligodendroglial FGFR2 in MOG 35‐55 ‐induced EAE. Oligodendrocyte‐specific knockout of FGFR2 ( Fgfr2 ind −/− ) was achieved by application of tamoxifen; EAE was induced using the MOG 35‐55 peptide. EAE symptoms were monitored over 62 days. Spinal cord tissue was analysed by histology, immunohistochemistry and western blot. Fgfr2 ind −/− mice revealed a milder disease course, less myelin damage and enhanced axonal density. The number of oligodendrocytes was not affected in demyelinated areas. However, protein expression of FGFR2, FGF2 and FGF9 was downregulated in Fgfr2 ind −/− mice. FGF/FGFR dependent signalling proteins were differentially regulated; pAkt was upregulated and pERK was downregulated in Fgfr2 ind −/− mice. The number of CD3(+) T cells, Mac3(+) cells and B220(+) B cells was less in demyelinated lesions of Fgfr2 ind −/− mice. Furthermore, expression of IL‐1β, TNF‐α and CD200 was less in Fgfr2 ind −/− mice than controls. Fgfr2 ind −/− mice showed an upregulation of PLP and downregulation of the remyelination inhibitors SEMA3A and TGF‐β expression. These data suggest that cell‐specific deletion of FGFR2 in oligodendrocytes has anti‐inflammatory and neuroprotective effects accompanied by changes in FGF/FGFR dependent signalling, inflammatory cytokines and expression of remyelination inhibitors. Thus, FGFRs in oligodendrocytes may represent potential targets for the treatment of inflammatory and demyelinating diseases including MS.