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A comprehensive library of fluorescent constructs of SARS‐CoV‐2 proteins and their initial characterisation in different cell types
Author(s) -
MisereyLenkei Stéphanie,
Trajkovic Katarina,
D'Ambrosio Juan Martín,
Patel Amanda J,
Čopič Alenka,
Mathur Pallavi,
Schauer Kristine,
Goud Bruno,
Albanèse Véronique,
Gautier Romain,
Subra Melody,
Kovacs David,
Barelli Hélène,
Antonny Bruno
Publication year - 2021
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1111/boc.202000158
Subject(s) - biology , centrosome , microbiology and biotechnology , yeast , endosome , cell , computational biology , biochemistry , cell cycle
Background Information Comprehensive libraries of plasmids for SARS‐CoV‐2 proteins with various tags ( e.g ., Strep, HA, Turbo) are now available. They enable the identification of numerous potential protein–protein interactions between the SARS‐CoV‐2 virus and host proteins. Results We present here a large library of SARS CoV‐2 protein constructs fused with green and red fluorescent proteins and their initial characterisation in various human cell lines including lung epithelial cell models (A549, BEAS‐2B), as well as in budding yeast. The localisation of a few SARS‐CoV‐2 proteins matches their proposed interactions with host proteins. These include the localisation of Nsp13 to the centrosome, Orf3a to late endosomes and Orf9b to mitochondria. Conclusions and Significance This library should facilitate further cellular investigations, notably by imaging techniques.