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miRNA 146b mediates the regulation of nucleolar size and activity in polyploid megakaryocytes
Author(s) -
Chattopadhyaya Saran,
Banerjee Subrata
Publication year - 2021
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1111/boc.202000022
Subject(s) - biology , nucleolus , endoreduplication , microbiology and biotechnology , ribosome biogenesis , polyploid , nucleolin , cell cycle , biogenesis , cytoplasm , genetics , cell , gene , ploidy , rna , ribosome
Background Information Megakaryocytes (MKs) follow a unique cell cycle duplication process, called endomitosis, resulting in polyploidisation of cells. It is hypothesised that polyploidy, as well as an increment in cytoplasm volume, allow more efficient platelets generation from MKs. Although polyploidy leads to an increase in the DNA amount, which impacts gene expression, little is known about ribosomal biogenesis in these polylobulated polyploid cells. Results The nucleolus acts as a hub for ribosomal biogenesis, which in turn governs the protein synthesis rate of the cells. We therefore estimated the size and activity of the nucleolus in polyploid cells during megakaryopoiesis in vitro . Polyploid megakaryocytic cell lines and in vitro cultured MKs, which were obtained from human cord blood‐derived CD 34 + cells, revealed that miRNA 146b regulated the activity of nucleolar and coiled‐body phosphoprotein 1, which plays an integral role in determining nucleolar size and activity. Additionally, miRNA‐146b was up‐regulated during endomitosis and was found to promote megakaryopoiesis. Conclusion We propose that miRNA 146b regulates not only nucleolar size and activity, but also megakaryopoiesis. Significance This study highlights the importance of nucleolar activity and miRNA in the progression of megakaryopoiesis and thrombopoiesis.