Premium
REV‐ERBα activates the mTOR signalling pathway and promotes myotubes differentiation
Author(s) -
DadonFreiberg Maayan,
Chapnik Nava,
Froy Oren
Publication year - 2020
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1111/boc.201900091
Subject(s) - mtorc1 , myogenesis , c2c12 , microbiology and biotechnology , myogenin , pi3k/akt/mtor pathway , biology , myocyte , cellular differentiation , mechanistic target of rapamycin , gene silencing , mtorc2 , signal transduction , biochemistry , gene
Background Information Mammalian target of rapamycin (mTOR) complex 1 (mTORC1) is a master regulator of cell and whole‐body energy homoeostasis. REV‐ERBα is a nuclear receptor that plays an important role in metabolism. While mTORC1 activation is necessary for muscle differentiation, the role of REV‐ERBα is less clear. Results We studied the effect of REV‐ERBα overexpression and silencing as well as mTORC1 activation and inhibition on the differentiation of C2C12 myoblasts to myotubes. mTOR, myogenin and REV‐ERBα were induced during differentiation of myoblasts into myotubes. REV‐ERBα was found to activate mTORC1 during the differentiation process even in the absence of the differentiation medium. This activation was presumably through the downregulation of the expression of TSC1, an mTORC1 inhibitor. Conclusion Herein we show that REV‐ERBα promotes myoblasts differentiation via the activation of the mTORC1 signalling pathway. Significance REV‐ERBα modulation can activate mTORC1 signalling and promote myoblasts differentiation.