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Rsph9 is critical for ciliary radial spoke assembly and central pair microtubule stability
Author(s) -
Zhu Lei,
Liu Hao,
Chen Yawen,
Yan Xiumin,
Zhu Xueliang
Publication year - 2019
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1111/boc.201800060
Subject(s) - cilium , biology , motile cilium , primary ciliary dyskinesia , microtubule , microbiology and biotechnology , ciliopathy , ciliogenesis , ependymal cell , flagellum , protein subunit , basal body , anatomy , neuroscience , genetics , phenotype , central nervous system , gene , medicine , bronchiectasis , lung
Background Information In the “9+2”‐type motile cilia, radial spokes (RSs) protruded from the nine peripheral microtubule doublets surround and interact with the central pair (CP) apparatus to regulate ciliary beat. RSPH9 is the human homologue of the essential protozoan RS head protein Rsp9. Its mutations in human primary ciliary dyskinesia patients, however, cause CP loss in a small portion of airway cilia without affecting the ciliary localization of other head proteins. Results We characterized mouse Rsph9 and investigated its function in ependymal motile cilia. Rsph9 was specifically expressed in mouse tissues containing motile cilia and upregulated during multiciliation. Its ciliary localization complied with its putative role as an RS subunit. Depletion of Rsph9 by RNAi in mouse ependymal cilia resulted in a near complete CP loss and altered the ciliary beat pattern from planar to rotational. Multiple RS proteins, including those in the head, were also markedly downregulated in the Rsph9‐depleted cilia. Conclusion Rsph9 is essential for both the RS head assembly and the CP maintenance in mammalian ependymal cilia. Significance Our results help to understand the assembly and functions of mammalian RS and pathology of RS‐related ciliopathy.