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Variation in traction forces during cell cycle progression
Author(s) -
Vianay Benoit,
Senger Fabrice,
Alamos Simon,
AnjurDietrich Maya,
Bearce Elizabeth,
Cheeseman Bevan,
Lee Lisa,
Théry Manuel
Publication year - 2018
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1111/boc.201800006
Subject(s) - contractility , tractive force , microbiology and biotechnology , cell cycle , biology , cell , morphogenesis , cell growth , traction (geology) , cell division , intracellular , endocrinology , genetics , paleontology , gene , structural engineering , engineering
Background Information Tissue morphogenesis results from the interplay between cell growth and mechanical forces. While the impact of geometrical confinement and mechanical forces on cell proliferation has been fairly well characterised, the inverse relationship is much less understood. Here, we investigated how traction forces vary during cell cycle progression. Results Cell shape was constrained on micropatterned substrates in order to distinguish variations in cell contractility from cell size increase. We performed traction force measurements of asynchronously dividing cells expressing a cell‐cycle reporter, to obtain measurements of contractile forces generated during cell division. We found that forces tend to increase as cells progress through G1, before reaching a plateau in S phase, and then decline during G2. Conclusions While cell size increases regularly during cell cycle progression, traction forces follow a biphasic behaviour based on specific and opposite regulation of cell contractility during early and late growth phases. Significance These results highlight the key role of cellular signalling in the regulation of cell contractility, independently of cell size and shape. Non‐monotonous variations of cell contractility during cell cycle progression are likely to impact the mechanical regulation of tissue homoeostasis in a complex and non‐linear manner.