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Flotillins control zebrafish epiboly through their role in cadherin‐mediated cell–cell adhesion
Author(s) -
Morris Eduardo A. Rios,
Bodin Stéphane,
Delaval Bénédicte,
Comunale Franck,
Georget Virginie,
Costa Manoel L.,
Lutfalla Georges,
GauthierRouvière Cécile
Publication year - 2017
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1111/boc.201700001
Subject(s) - epiboly , zebrafish , biology , cadherin , microbiology and biotechnology , gastrulation , cell , gene knockdown , cell adhesion , cell migration , embryo , gene , genetics , embryogenesis
Zebrafish gastrulation and particularly epiboly that involves coordinated movements of several cell layers is a dynamic process for which regulators remain to be identified. We show here that Flotillin 1 and 2, ubiquitous and highly conserved proteins, are required for epiboly. Flotillins knockdown compromised embryo survival, strongly delayed epiboly and impaired deep cell radial intercalation and directed collective migration without affecting enveloping layer cell movement. At the molecular level, we identified that Flotillins are required for the formation of E‐cadherin‐mediated cell–cell junctions. These results provide the first in vivo evidence that Flotillins regulate E‐cadherin‐mediated cell–cell junctions to allow epiboly progression.