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Publication year - 2016
Publication title -
biology of the cell
Language(s) - English
Resource type - Reports
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1111/boc.201570037
Subject(s) - biology , microbiology and biotechnology , phalloidin , sted microscopy , dapi , cytoskeleton , vacuole , confocal microscopy , efferocytosis , phagosome , actin , cytoplasm , apoptosis , cell , phagocytosis , biochemistry , superresolution , macrophage , in vitro , artificial intelligence , computer science , image (mathematics)
The underlying mechanisms of efferocytosis by fibroblasts were not well understood. We demonstrate that the engulfment of PKH67‐labeled ( green ) apoptotic cells by human primary fibroblasts (HPFs) requires the remodeling of the actin cytoskeleton labeled with tetramethylrhodamine‐B‐isothiocyanate‐conjugated phalloidin ( red ). Confocal microscopy imaging reveals the typical features of phagosome formation that ultimately results in the engulfment of the apoptotic cell in a membrane‐bound vacuole. The nuclei are stained with DAPI ( blue ).
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