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Optogenetic reporters
Author(s) -
Alford Spencer C.,
Wu Jiahui,
Zhao Yongxin,
Campbell Robert E.,
Knöpfel Thomas
Publication year - 2013
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1111/boc.201200054
Subject(s) - optogenetics , biology , computational biology , transduction (biophysics) , genetically modified organism , fluorescent protein , neuroscience , microbiology and biotechnology , green fluorescent protein , genetics , biophysics , gene
The discovery of naturally evolved fluorescent proteins and their subsequent tuning by protein engineering provided the basis for a large family of genetically encoded biosensors that report a variety of physicochemical processes occurring in living tissue. These optogenetic reporters are powerful tools for live‐cell microscopy and quantitative analysis at the subcellular level. In this review, we present an overview of the transduction mechanisms that have been exploited for engineering these genetically encoded reporters. Finally, we discuss current and future efforts towards the combined use of various optogenetic actuators and reporters for simultaneously controlling and imaging the physiology of cells and tissues.