Premium
Sdf‐1 (CXCL12) improves skeletal muscle regeneration via the mobilisation of Cxcr4 and CD34 expressing cells
Author(s) -
Brzoska Edyta,
Kowalewska Magdalena,
MarkowskaZagrajek Agnieszka,
Kowalski Kamil,
Archacka Karolina,
Zimowska Małgorzata,
Grabowska Iwona,
Czerwińska Areta M.,
CzarneckaGóra Magdalena,
Stremińska Władysława,
JańczykIlach Katarzyna,
Ciemerych Maria A.
Publication year - 2012
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1111/boc.201200022
Subject(s) - biology , myocyte , microbiology and biotechnology , regeneration (biology) , skeletal muscle , progenitor cell , stem cell , cd34 , stromal cell , extracellular matrix , myogenesis , endocrinology , cancer research
Background information The regeneration of skeletal muscles involves satellite cells, which are muscle‐specific precursor cells. In muscles, injured either mechanically or as a consequence of a disease, such as muscular dystrophy, local release of the growth factors and cytokines leads to satellite cells activation, proliferation and differentiation of the resulting myoblasts, followed by the formation of new myofibres. Various cell types, such as stem and progenitor cells, originating from other tissues different than the muscle, are also able to follow a myogenic program. Participation of these cells in the repair process depends on their precise mobilisation to the site of the injury. Results In this study, we showed that stromal‐derived factor‐1 (Sdf‐1) impacts on the mobilisation of CXC chemokine receptor (Cxcr)4‐positive cells and improves skeletal muscle regeneration. Analysis of isolated and in vitro cultured satellite cells showed that Sdf‐1 did not influence myoblasts proliferation and expression of myogenic regulatory transcription factors but induced migration of the myoblasts in Cxcr4‐dependent ways. This phenomenon was also associated with the increased activity of crucial extracellular matrix modifiers, i.e. metalloproteases Mmp‐2 and Mmp‐9. Conclusions Thus, positive impact of Sdf‐1 on muscle regeneration is related to the mobilisation of endogenous cells, that is satellite cells and myoblasts, as well as non‐muscle stem cells, expressing Cxcr4 and CD34.