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Chronic nonbacterial prostate inflammation in a rat model is associated with changes of gut microbiota that can be modified with a galactoglucomannan‐rich hemicellulose extract in the diet
Author(s) -
Konkol Yvonne,
Keskitalo Anniina,
Vuorikoski Heikki,
Pietilä Sami,
Elo Laura L.,
Munukka Eveliina,
Bernoulli Jenni,
Tuomela Johanna
Publication year - 2019
Publication title -
bju international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.773
H-Index - 148
eISSN - 1464-410X
pISSN - 1464-4096
DOI - 10.1111/bju.14553
Subject(s) - lachnospiraceae , gut flora , butyric acid , lipopolysaccharide binding protein , bacteroides , food science , clostridium , hemicellulose , lactobacillus , clostridiaceae , biology , endocrinology , medicine , microbiology and biotechnology , firmicutes , biochemistry , inflammation , bacteria , 16s ribosomal rna , fermentation , acute phase protein , toxin , genetics , gene , hydrolysis
Objectives To investigate dietary effects on the gut microbiota composition in a rat model of nonbacterial chronic prostate inflammation (CPI). Materials and methods Nonbacterial CPI was induced in the Wistar rat strain with subcutaneous testosterone and 17β‐oestradiol (E 2 ) hormone pellets for 18 weeks. Rats with placebo pellets served as healthy controls. Rats with CPI were stratified into two groups, which drank either plain tap water (control group) or tap water supplemented with 2% galactoglucomannan‐rich hemicellulose extract ( GGM group) from Norway spruce ( Picea abies ) for 5 weeks. Faecal samples were collected at the end of the study, total DNA was extracted, and the bacterial composition was analysed by 16S rRNA gene sequencing. In addition, faecal samples were assayed for short‐chain fatty acid ( SCFA ) concentrations using gas chromatography. Lipopolysaccharide‐binding protein ( LBP ) was measured in serum samples, as an indirect indicator for bacterial lipopolysaccharide (LPS) load in blood. Results The microbial biodiversity was significantly different between the treatment groups. In the rats with CPI, there was a significant increase in gut microbial populations Rikenellaceae , Odoribacter , Clostridiaceae, Allobaculum and Peptococcaceae compared with healthy rats. Conversely, levels of Bacteroides uniformis , Lactobacillus and Lachnospiraceae were decreased in rats with CPI. SCFA butyric‐, valeric‐ and caproic‐acid concentrations were also decreased in the faecal samples of the rats with CPI. In contrast, acetic acid concentrations and serum LBP were significantly elevated in CPI rats compared with healthy ones. Amongst rats with CPI, treatment with the GGM extract significantly reduced the abundance of Odoribacter and Clostridiaceae levels, and increased the B. uniformis levels compared with CPI rats drinking tap water only. In addition, GGM significantly increased the levels of butyric acid and caproic acid, and reduced the levels of LBP in serum. Conclusions Hormone‐induced nonbacterial CPI in rats is associated with specific changes in gut microbiota and secondary changes in SCFA s and LPS due to gut microbiota alteration. Our results further suggest that fermentable compounds may have a beneficial effect on CPI.