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Immunocytochemical detection of ERG expression in exfoliated urinary cells identifies with high specificity patients with prostate cancer
Author(s) -
Pal Raj P.,
Kockelbergh Roger C.,
Pringle John Howard,
Cresswell Lara,
Hew Roger,
Dormer John P.,
Cooper Colin,
Mellon John Kilian,
Barwell Julian G.,
Hollox Edward J.
Publication year - 2016
Publication title -
bju international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.773
H-Index - 148
eISSN - 1464-410X
pISSN - 1464-4096
DOI - 10.1111/bju.13184
Subject(s) - erg , prostate cancer , prostate , immunohistochemistry , tmprss2 , pathology , medicine , biopsy , urinary system , prostate specific antigen , pca3 , cancer , biology , disease , ophthalmology , retinal , covid-19 , infectious disease (medical specialty)
Objectives To evaluate the immunocytochemical detection of ERG protein in exfoliated cells as a means of identifying patients with prostate cancer ( PC a) before prostate biopsy. Materials and Methods Urine samples (30 mL) were collected after digital rectal examination ( DRE ) from 159 patients with an elevated age‐specific prostate‐specific antigen ( PSA ) and/or an abnormal DRE who underwent prostate biopsy. In all cases, exfoliated urinary cells from half of the urine sample underwent immunocytochemical assessment for ERG protein expression. Exfoliated cells in the remaining half underwent assessment of TMPRSS 2: ERG status using either nested reverse‐transcriptase ( RT )‐ PCR (151 cases) or fluorescence in situ hybridization ( FISH ; eight cases). Corresponding tissue samples were evaluated using FISH to determine chromosomal gene fusion tissue status and immunohistochemistry ( IHC ) to determine ERG protein expression. Results were correlated with clinicopathological variables. Results The sensitivity and specificity of urinary ERG immunocytochemistry ( ICC ) for PC a were 22.7 and 100%, respectively. ERG ICC results correlated with advanced tumour grade, stage and higher serum PSA . In comparison, urine TMPRSS 2: ERG transcript analysis had 27% sensitivity and 98% specificity for PC a detection. On tissue IHC , ERG staining was highly specific for PC a. In all, 52% of cancers harboured foci of ERG staining; however, only 46% of cancers that were found to have ERG overexpression were positive on urine ICC . The ERG ICC results showed strong concordance with urinary RT ‐ PCR and FISH , and tissue IHC and FISH . Conclusion This is the first study to show that cytological gene fusion detection using ICC is feasible and identifies patients with adverse disease markers. ERG ICC was highly specific, but this technique was less sensitive than RT ‐ PCR .