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Biodistribution of Evans blue in an orthotopic AY ‐27 rat bladder urothelial cell carcinoma model: implication for the improved diagnosis of non‐muscle‐invasive bladder cancer ( NMIBC ) using dye‐guided white‐light cystoscopy
Author(s) -
Elsen Sanne,
Lerut Evelyne,
Van Cleynenbreugel Ben,
Aa Frank,
Poppel Hein,
Witte Peter A.
Publication year - 2015
Publication title -
bju international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.773
H-Index - 148
eISSN - 1464-410X
pISSN - 1464-4096
DOI - 10.1111/bju.13113
Subject(s) - urothelium , urothelial cell , pathology , bladder cancer , evans blue , urinary bladder , submucosa , biodistribution , immunohistochemistry , tight junction , adherens junction , chemistry , frozen section procedure , cancer , cell , biology , medicine , urology , microbiology and biotechnology , cadherin , endocrinology , biochemistry , in vitro
Objectives To investigate the possibility of using Evans blue ( EB ) as a novel diagnostic tool to detect bladder tumours with white‐light ( WL ) cystoscopy, in this preclinical study we examine the biodistribution of EB in the different layers (urothelium, submucosa, muscle) of a normal rat bladder and a rat bladder bearing a malignant urothelium composed of syngeneic AY ‐27 tumour cells. Materials and Methods EB was instilled into both normal as well as tumour‐bearing rat bladders. After instillation, bladders were removed and snap frozen in liquid nitrogen. The distribution of EB in the different layers was quantified using fluorescence microscopy. To gain more insight into the mechanism underlying the selective accumulation of EB in tumour tissue, bladder sections were prepared for ultrastructural investigations by means of transmission electron microscopy ( TEM ). In addition, we also examined the expression of E‐cadherin, claudin‐1 and desmoglein‐1 by immunohistochemistry to study the integrity of the bladder wall, as these molecules are key constituents of adherens junctions, tight junctions and desmosomes, respectively. Results In most cases, the accumulation of EB in malignant bladders was substantially higher than in healthy bladders, at least when 1 m m EB instillations were used. In case of a 1 m m EB instillation for 2 h, the EB ‐associated fluorescence in malignant urothelial tissue was 55‐times higher than the fluorescence found in normal urothelium. Ultrastructurally, malignant tissue displayed wider intercellular spaces and a decreased number of cell junction components compared with normal tissue, pointing to defects in the urothelial barrier. There were no differences in the expression of E‐cadherin, whereas desmoglein‐1 staining was stronger in the membranes of healthy bladder urothelium compared with tumour tissue. Claudin‐1 expression was negative in all samples tested. Conclusion EB is selectively taken up by tumour tissue after intravesical instillations in rats bearing bladder tumours. The lower expression of desmoglein‐1 in tumour samples, together with the reduced presence of desmosomes seen with TEM , likely imply that desmosomes play an important role in the ultrastructural differences between healthy rat urothelium and tumour tissue, and secondary to that, to the differential uptake of EB in both tissues. We consider that our findings could be useful for future clinical developments in the field of diagnostics for bladder cancer.